16s ribosomal rna gene pyrosequencing  (Pyrosequencing Inc)

Journal: BMC Nephrology

Article Title: The gut microbiota profile of adults with kidney disease and kidney stones: a systematic review of the literature

doi: 10.1186/s12882-020-01805-w

Figure Lengend Snippet: Characteristics of included studies

Article Snippet: Shi 2014 [ ] , China , N = 52 n = 22 HD group Dialysis vintage: 6-40 months, Kt/V not reported. n = 30 reported ESKD not undertaking dialysis. eGFR, age, gender and ethnicity not reported. , N = 10 eGFR, age, gender and ethnicity not reported. , Details of sample collection not reported; Samples immersed in 90% alcohol and stored at -20°C , Excluded if taken in prior 3 weeks. , Medications not reported; Corticosteroids, statins, cytotoxic drugs not taken in prior 3 weeks. , SILVA , 16S ribosomal RNA gene Pyrosequencing (V1-V3 region) Sequencing platform not reported , ↑ α-diversity in HD group vs. controls (average OTUs/ species, p =0.044) and Chao1 index higher in three HD samples ( p -value not reported). β-diversity not reported between study groups. However, PCA plot of Unifrac data from selected samples at the genus level exhibited a large separation in the same three HD samples vs. the other selected samples ( p -value or statistical test not report). , Not reported.

Techniques: Medications, Sequencing, Control, Amplification, Real-time Polymerase Chain Reaction, Generated, Microarray, Sterility, Sampling, Transplantation Assay